FastQCFastQC Report
Fri 13 Nov 2020
test_rg_3.bam

Summary

[OK]Basic Statistics

MeasureValue
Filenametest_rg_3.bam
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATCCCCTACATCGAGACCTCGGCCAAGACCCGGCAGGTGAGGCAGCTC21.1764705882352942No Hit
GGCCAGCCTCACGGGGTTCACCTGTACTGGTGGATGTCCTCAAAAGACTT21.1764705882352942No Hit
TGCCGTAGCTTCGGGCGAGGTCCTGAGCCTGCCGAGATTCCACAGTGCGT21.1764705882352942No Hit
CGTCATCCGAGTCCTTCACCCGTTTGATCTGCTCCCTGAGAGGTGGAAAG21.1764705882352942No Hit
GCAGCTCTCCACCCCACAGCTAGCCAGGGACCCGCCCCGCCCCGCCCCAG21.1764705882352942No Hit
TGGGGTCGTATTCGTCCACAAAGTGGTTCTGGATCAGCTGGATGGTCAGC21.1764705882352942No Hit
TGAGAGGTACCAGGGAGAGGCTGGCTGTGTGAACTCCCCCCACGGAAGGT21.1764705882352942No Hit
GAGCCAGAGCGGCTGCCCTGTGTCAAGGGAGAGGGTCAGTGAGTGCTGCT21.1764705882352942No Hit
GAGCAGATCAAACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGT21.1764705882352942No Hit
GTGGTGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCGCTGACCATCCAGCT21.1764705882352942No Hit
CCCGGAGCTGGAGCTAGAGCCAGAGCGGCTGCCCTGTGTCAAGGGAGAGG21.1764705882352942No Hit
GGGGCCTGGGCCTGGCTGAGCAGGGCCCTCCTTGGCAGGTGGGGCAGGAG10.5882352941176471No Hit
ATCCAGCTGATCCAGAACCACTTTGTGGACGAATACGACCCCACTATAGA10.5882352941176471No Hit
CACTGTGGAATCTCGGCAGGCCCAGGACCTCGCCCGAAGCTACGGCATCC10.5882352941176471No Hit
TCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGTGAACCCCG10.5882352941176471No Hit
GGCGGTATCCAGGATGTCCAACAGGCACGTCTCCCCATCAATGACCACCT10.5882352941176471No Hit
TCTATAGTGGGGTCGTATTCGTCCACAAAGGGGTTCTGGATCAGCTGGAT10.5882352941176471No Hit
CTCACCTGCGTCAGGAGAGCACACACTTGCAGCTCATGCAGCCGGGGCCA10.5882352941176471No Hit
GTATCCAGGATGTCCAACAGGCACGTCTCCCCATCAATGACCACCTGCTT10.5882352941176471No Hit
TGAGCCCAGACCCCGGCCCTCGCCTCCCTCACTGCCCTGCCGTCCCGGGA10.5882352941176471No Hit
GGGGCGGGGCGGGTCCCTGGCTAGCTGTGGGGTGGAGAGCTGCCTCACCT10.5882352941176471No Hit
TGCCGGGTCTTGGCCGAGGTCTCGATGTAGGGGATGCCGTAGCTTCGGGC10.5882352941176471No Hit
CCTGTTGGACATCCTGGATACCGCCGGCCAGGAGGAGTACAGCGCCATGC10.5882352941176471No Hit
GCACCGGGGAGGGCTTCCTGTGTGTGTTTGCCATCAACAACACCAAGTCT10.5882352941176471No Hit
CATGTCCTGGATGCCGCTGCGCCTGCAGCCCCCGTAGCCAGCTCTCGCTT10.5882352941176471No Hit
CCTGTGTGTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACC10.5882352941176471No Hit
AGCCAGCTCTCGCTTTCCACCTCTCAGGGAGCAGATCAAACGGGTGAAGG10.5882352941176471No Hit
TGTGTGAACTCCCCCCACGGAAGGTCCTGAGGGGGTCCCTGAGCCCTGTC10.5882352941176471No Hit
CCGTAGCCAGCTCTCGCTTTCCACCTCTCAGGGAGCAGATCAAACGGGTG10.5882352941176471No Hit
CTGCAGCCCCCGTAGCCAGCTCTCGCTTTCCACCTCTCAGGGAGCAGATC10.5882352941176471No Hit
TGTGAACTCCCCCCACGGAAGGTCCTGAGGGGGTCCCTAAGCCCTGTCCT10.5882352941176471No Hit
GGCTGGCTGTGTGAACTCCCCCCACGGAAGGTCCTGAGGGGGTCCCTGAG10.5882352941176471No Hit
GCCTGCCGAGATTCCACAGTGCGTGCAGCCAGGTCACACTTGTTCCCCAC10.5882352941176471No Hit
GACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGTGTGTTTGCCATCAA10.5882352941176471No Hit
TGGCCCCACCTGTGCGGCGTGGGCTCCCGGGCCAGCCTCACGGGGTTCAC10.5882352941176471No Hit
CCCTGAGGAGCGATGACGGAATATAAGCTGGTGGTGGTGGGCGCCGGCGG10.5882352941176471No Hit
CATCCAGGACATGCGCAGAGAGGACAGGAGGCCCCTGCCTGGACGCAGCC10.5882352941176471No Hit
AGGGAGAGGCTGGCTGTGTGAACTCCCCCCACGGAAGGTCCTGAGGGGGT10.5882352941176471No Hit
CCTCCTGTCCTCTCTGCGCATGTCCTGGATGCCGCTGCGCCTGCAGCCCC10.5882352941176471No Hit
ATGGGTCCCGGGGGGTCCCAGAGGGTCCCGGAGCTGGAGCTAGAGCCAGA10.5882352941176471No Hit
GCTAGCTGTGGGGTGGAGAGCTGCCTCACCTGCCGGGTCTTGGCCGAGGT10.5882352941176471No Hit
GACATCCTGGATACCGCCGGCCAGGAGGAGTACAGCGCCATGCGGGACCA10.5882352941176471No Hit
CGTAGCTTCGGGCGAGGTCCTGAGCCTGCCGAGATTCCACAGTGCGTGCA10.5882352941176471No Hit
CAACACCAAGTCTTTTGAGGACATCCACCAGTACAGGTGAACCCCGTGAG10.5882352941176471No Hit
CGGGTCTTGGCCGAGGTCTCGATGTAGGGGATGCCGTAGCTTCGGGCGAG10.5882352941176471No Hit
GCACGCACTGTGGAATCTCGGCAGGCTCAGGACCTCGCCCGAAGCTACGG10.5882352941176471No Hit
CGCATGGCGCTGTACTCCTCCTGGCCGGCGGTATCCAGGATGTCCAACAG10.5882352941176471No Hit
TTATAAGTCGCCGGGGGCTCGGGTTCCGTGCGGCTGAGCCACGCCCCTTA10.5882352941176471No Hit
AGAGCACACACTTGCAGCTCATGCAGCCGGGGCCACTCTCATCAGGAGGG10.5882352941176471No Hit
GCGCAGCGGCATCCAGGACATGCGCAGAGAGGACAGGAGGCCCCTGCCTG10.5882352941176471No Hit
AACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAG10.5882352941176471No Hit
GTGGGGCCTGGGCCTGGCTGAGCAGGGCCCTCCTTGGCAGGTGGGGCAGG10.5882352941176471No Hit
CACCTGCTTCCGGTAGGAATCCTGCAGGAGGACAGGGCTCAGGGACCCCC10.5882352941176471No Hit
CAGGCACGTCTCCCCATCAATGACCACCTGCTTCCGGTAGGAATCCTGCA10.5882352941176471No Hit
CTGATCCCATCCCTCCTTTCCCAGGGAGTGGAGGATGCCTTCTACACGTT10.5882352941176471No Hit
CGGGCGAGGTCCTGAGCCTGCCGAGATTCCACAGTGCGTGCAGCCAGGTC10.5882352941176471No Hit
CGCCTGCAGCCCCCGTAGCCAGCTCTCGCTTTCCACCTCTCAGGGAGCAG10.5882352941176471No Hit
GGCCGAGGTCTCGATGTAGGGGATGCCGTAGCTTCGGGCGAGGTCCTGAG10.5882352941176471No Hit
AGTGAGTGCTGCTCCCTGGCTGGGGCGGGGCGGGGCGGGCCCCTTGCGAG10.5882352941176471No Hit
GCGTCCATGCAGGGGCCTCCTGTCCTCTCTGCGCATGTCCTGGATGCCGC10.5882352941176471No Hit
GCATGTACTGGTCCCGCATGGCGCTGTACTCCTCCTGGCCGGAGGTATCC10.5882352941176471No Hit
TGCCTGGACGCAGCCGGCCTGGCCCCACCTGGGCGGCGTGGGCTCCCGGG10.5882352941176471No Hit
TGACCACCTGCTTCCGGTAGGAATCCTGCAGGAGGACAGGGCTCAGGGAC10.5882352941176471No Hit
TGCATGGTGCTGTGGCCCTGTCTCCTGCTTCCTCTAGAGGAGGGGAGTCC10.5882352941176471No Hit
GAAGTGGCTGGGGGAGTCGGGAGCTTCGGGCCAGGCAAGGCTTGATCCCA10.5882352941176471No Hit
CACCTGCGTCAGGAGAGCACACACTTGCAGCTCATGCAGCCGGGGCCACT10.5882352941176471No Hit
GTGCGCATGTACTGGTCCCGCATGGCGCTGTACTCCTCCTGGCCGGCGGT10.5882352941176471No Hit
ATTCCTACCGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCTGTTGGAC10.5882352941176471No Hit
AGGGCCACAGCACCATGCAGGGGACCAGGGGCTGCAGCCAGCCCTATCCT10.5882352941176471No Hit
CCCCTGCCTGGACGCAGCCGGCCTGGCCCCACCTGTGCGGCGTGGGCTCC10.5882352941176471No Hit
GGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGCGCTTCC10.5882352941176471No Hit
CTCACGGGGTTCACCTGTACTGGTGGATGTCCTCAAAAGACTTGGTGTTG10.5882352941176471No Hit
CGGGGTCATCCGGTGGGCGTGGCGGACGCCCTGGGAGTCCCCCTCACCTG10.5882352941176471No Hit
CCCACCAGCACCATGGGCACGTCATCCGAGTCCTCACCCGTTTGATCTGC10.5882352941176471No Hit
GGGTCCCGGGGGGTCCCAGAGGGTCCCGGAGCTGGAGCTAGAGCCAGAGC10.5882352941176471No Hit
AGCTGCCTCACCTGCCGGGTCTTGGCCGAGGTCTCGATGTAGGGGATGCC10.5882352941176471No Hit
ATCCAGGATGTCCAACAGGCACGTCTCCCCATCAATGACCACCTGCTTCC10.5882352941176471No Hit
CGGGGAGGGCTTCCTGTGTGTGTTTGCCATCAACAACACCAAGTCTTTTG10.5882352941176471No Hit
CACCTTCCCCTGGGATCGCCCGCAGCAGGGGCGCCCACGCACGTGCCAGC10.5882352941176471No Hit
AGCCCTCCCCGGTGCGCATGTACTGGTCCCGCATGGCGCTGTACTCCTCC10.5882352941176471No Hit
ATTGATGGGGAGACGTGCCTGTTGGACATCCTGGATACCGCCGGCCAGGA10.5882352941176471No Hit
GCGTGAGCCCAGACCCCGGCCCTCGCCTCCCTCACTGCCCTGCCGTCCCG10.5882352941176471No Hit
ATGCCGTAGCTTCGGGCGAGGTCCTGAGCCTGCCGAGATTCCACAGTGCG10.5882352941176471No Hit
TGCGCATGTACTGGTCCCGCATGGCGCTGTACTCCTCCTGGCCGGCGGTA10.5882352941176471No Hit
GTGAACTCCCCCCACGGAAGGTCCTGAGGGGGTCCCTGAGCCCTGTCCTC10.5882352941176471No Hit
CATGTACTGGTCCCGCATGGCGCTGTACTCCTCCTGGCCGGCGGTATCCA10.5882352941176471No Hit
CCCACCTGTGCGGCGTGGGCTCCCGGGCCAGCCTCACGGGGTTCACCTGT10.5882352941176471No Hit
TGACCTGGCTGCACGCACTGTGGAATCTCGGCAGGCTCAGGACCTCGCCC10.5882352941176471No Hit
GCAGCAGCTGCTGGCACCTGGACGGCGGCGCCAGGCTCACCTCTATAGTG10.5882352941176471No Hit
CCCGCATGGCGCTGTACTCCTCCTGGCCGGCGGTATCCAGGATGTCCAAC10.5882352941176471No Hit
ACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCCTGTGT10.5882352941176471No Hit
GTCAGTGAGTGCTGCTCCCTGGCTGGGGCGGGGCGGGGCGGGGCCCCGGC10.5882352941176471No Hit
TGGCCCGGGAGCCCACGCCGCACAGGTGGGGCCAGGCCGGCTGCGTCCAG10.5882352941176471No Hit
GTGTTTGCCATCAACAACACCAAGTCTTTTGAGGACATCCACCAGTACAG10.5882352941176471No Hit
CTGCAGGATTCCTACCGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCT10.5882352941176471No Hit
GCTGGTGGTGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCGCTGACCATCC10.5882352941176471No Hit
AGACAGGGCCACAGCACCATGCAGGGGACCAGGGGCTGCAGCCAGCCCTA10.5882352941176471No Hit
CAGGCTCAGGACCTCGCCCGAAGCTACGGCATCCCCTACATCGAGACCTC10.5882352941176471No Hit
AACTCCCCCCACGGAAGGTCCTGAGGGGGTCCCTGAGCCCTGTCCTCCTG10.5882352941176471No Hit
CTCGTCTCAGCACCCCAGGAGAGGAGGGGGCATGAGGGGCATGAGAGGTA10.5882352941176471No Hit
ACCTGCGTCAGGAGAGCACACACTTGCAGCTCATGCAGCCGGGGCCACTC10.5882352941176471No Hit
CCCATGGTGCTGGTGGGGAACAAGTGTGACCTGGCTGCACGCACTGTGGA10.5882352941176471No Hit
TAGCCAGGGACCCGCCCCGCCCCGCCCCAGCCAGGGAGCAGCACTCACTG10.5882352941176471No Hit
CCTGCCGGGTCTTGGCCCAGGTCTCGATGTAGGGGATGCCGTAGCTTCGG10.5882352941176471No Hit
GTAGCTTCGGGCGAGGTCCTGAGCCTGCCGAGATTCCACAGTGCGTGCAG10.5882352941176471No Hit
TGCCTGGACGCAGCCGGCCTGGCCCCACCTGTGCGGCGTGGGCTCCCGGG10.5882352941176471No Hit
TCCTCCTGGCCGGCGGTATCCAGGATGTCCAACAGGCACGTCTCCCCATC10.5882352941176471No Hit
CGGCAGGCTCAGGACCTCGCCCGAAGCTACGGCATCCCCTACATCGAGAC10.5882352941176471No Hit
CACAGGTGGGACCAGGCCGGCTGCGTCCAGGCAGGGGCCTCCTGTCCTCT10.5882352941176471No Hit
TCACCTGTACTGGTGGATGTCCTCAAAAGACTTGGTGTTGTTGATGGCAA10.5882352941176471No Hit
GCGTCCAGGCAGGGGCCTCCTGTCCTCTCTGCGCATGTCCTGGATGCCGC10.5882352941176471No Hit
TGGTGGGCGCCGGCGGTGTGGGCAAGAGTGCGCTGACCATCCAGCTGATC10.5882352941176471No Hit
ACGGGTGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAGT10.5882352941176471No Hit
CACCTCTCAGGGAGCAGATCAAACGGGTGAAGGACTCGGATGACGTGCCC10.5882352941176471No Hit
CAGGCAGGGGCCTCCTGTCCTCTCTGCGCATGTCCTGGATGCCGCTGCGC10.5882352941176471No Hit
ACCTGTGCGGCGTGGGCTCCCGGGCCAGCCTCACGGGGTTCACCTGTACT10.5882352941176471No Hit
CCTGATTCCTCTAGAGGAGGGGAGTCCCTCCTCTCAGCACCCCAGGCGTG10.5882352941176471No Hit
GATACCGCCGGCCAGGAGGAGTACAGCGCCATGCGGGACCAGTACATGCG10.5882352941176471No Hit
GAAGGCCCCTGAGGAGCGATGACGGAATATAAGCTGGTGGGGGGGGGCGC10.5882352941176471No Hit
GCCCACACCGCCGGCGCCCACCACCACCAGCTTATATTCCGTCATCGCTC10.5882352941176471No Hit
CACTGTGGAATCTCGGCAGGCTCAGGACCTCGCCCGAAGCTACGGCATCC10.5882352941176471No Hit
GATGTAGGGGATGCCGTAGCTTCGGGCGAGGTCCTGAGCCTGCCGAGATT10.5882352941176471No Hit
GGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGGGGAGGGCTTCC10.5882352941176471No Hit
AGCCAGGTCACACTTGTTCCCCACCAGCACCATGGGCACGTCATCCGAGT10.5882352941176471No Hit
TACTCCTCCTGGCCGGCGGTATCCAGGATGTCCAACAGGCACGTCTCCCC10.5882352941176471No Hit
GGCCACTCTCATCAGGAGGGTTCAGCTTCCGCAGCTTGTGCTGCCGGATC10.5882352941176471No Hit
TCCTACCGGAAGCAGGTGGTCATTGATGGGGAGACGTGCCTGTTGGACAT10.5882352941176471No Hit
GGCTGCCCTGTGTCAAGGGAGAGGGTCAGTGAGTGCTGCTCCCTGGCTGG10.5882352941176471No Hit
TGAAGGACTCGGATGACGTGCCCATGGTGCTGGTGGGGAACAAGTGTGAC10.5882352941176471No Hit
TGCGCCTGCAGCCCCCGTAGCCAGCTCTCGCTTTCCACCTCTCAGGGAGC10.5882352941176471No Hit
CTCCCCCCACGGAAGGTCCTGAGGGGGTCCCTGAGCCCTGTCCTCCTGCA10.5882352941176471No Hit
TCTAGAGGAAGCAGGAGACAGGGCCACAGCACCATGCAGGGGACCAGGGG10.5882352941176471No Hit
GCCGGCCAGGAGGAGTACAGCGCCATGCGGGACCAGTACATGCGCACCGG10.5882352941176471No Hit
CCTCACGGGGTTCACCTGTACTGGTGGATGTCCTCAAAAGACTTGGTGTT10.5882352941176471No Hit
GGTAGCTGTGGGGTGGAGAGCTGCCTCACCTGCCGGGTCTTGGCCGAGGT10.5882352941176471No Hit
TGTACTGGTGGATGTCCTCAAAAGACTTGGTGTTGTTGATGGCAAACACA10.5882352941176471No Hit
CCCGCCCCAGCCAGGGAGCAGCACTCACTGACCCTCTCCCTTGACACAGG10.5882352941176471No Hit
TGGAGGATGCCTTCTACACGTTGGTGCGTGAGATCCGGCAGCACAAGCTG10.5882352941176471No Hit
GTGCAGCCAGGTCACACTTGTTCCCCACCAGCACCATGGGCACGTCATCC10.5882352941176471No Hit
CCCACAGGAAGCCCTCCCCGGAGCGCATGTACTGGACCCGCATGGCGCTG10.5882352941176471No Hit
AGCTGGATGGTCAGCGCACTCTTGCCCACACCGCCGGCGCCCACCACCAC10.5882352941176471No Hit
GGAAGGTCCTGAGGGGGTCCCTGAGCCCTGTCCTCCTGCAGGATTCCTAC10.5882352941176471No Hit
CCACAGTGCGTGCAGCCAGGTCACACTTGTTCCCCACCAGCACCATGGGC10.5882352941176471No Hit
CACAGCAGGGAGCCCCTCACCCAGGCAGGCGGCCACAGGCCGGTCCCTCC10.5882352941176471No Hit
TCAAAAGACTTGGTGTTGTTGATGGCAAACACACACAGGAAGCCCTCCCC10.5882352941176471No Hit
ATCCACCAGTACAGGTGAACCCCGTGAGGCTGGCCCGGGAGCCCACGCCG10.5882352941176471No Hit
TCCACCCCACAGCTAGCCAGGGACCCGCCCCGCCCCGCCCCAGCCAGGGA10.5882352941176471No Hit
AAGCTAAGGCATCCCCTACATCGAGACCTCGGCCAAGACCCGGCAGGTGA10.5882352941176471No Hit
TGGGTCACATGGGTCCCGGGGGGTCCCAGAGGGTCCCGGAGCTGGAGCTA10.5882352941176471No Hit
AGGACATGCGCAGAGAGGACAGGAGGCCCCTGCCTGGACGCAGCCGGCCT10.5882352941176471No Hit
TGGCTGTGTGAACTCCCCCCACGGAAGGTCCTGAGGGGGTCCCTGAGCCC10.5882352941176471No Hit
GGAGTCGGGAGCTTCGGGCCAGGCAAGGCTTGATCCCACAGCAGGGAGCC10.5882352941176471No Hit
AATCTCGGCAGGCTCAGGACCTCGCCCGAAGCTACGGCATCCCCTACATC10.5882352941176471No Hit
GTTGATGGCAAACACACACAGGAAGCCCTCCCCGGTGCGCATGTACTGGT10.5882352941176471No Hit
GTCTTGGCCGAGGTCTCGATGTAGGGGATGCCGTAGCTTCGGGCGAGGTC10.5882352941176471No Hit
ACAGGCCGGTCCCTCCTGATCCCATCCCTCCTTTCCCAGGGAGTGGAGGA10.5882352941176471No Hit
CACCATGGGCACGTCATCCGAGTCCTACACCCGTTTGATCTGCTCCCTGA10.5882352941176471No Hit
TACCAGGGAGAGGCTGGCTGTGTGAACTCCCCCCACGGAAGGTCCTGAGG10.5882352941176471No Hit
TCTAGAGGAGGGGAGTCCCTCGTCTCAGCACCCCAGGAGAGGAGGGGGCA10.5882352941176471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)